This study reports on the preparation and characterization of porous clay heterostructure (PCH) as a high-surface area support for CuO nanoparticles based antibacterial activity for Gram-positive and Gram-negative bacteria.CuO nanoparticles were incorporated in PCH by thermal decomposition method and characterized by diffuse reflectance spectroscopy (DRS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption-desorption measurements (BET) and transmission electron microscopy (TEM), The TEM results Spare UHF batteries showed that nanoparticles are nearly spherical and particles size in the range of less than 10 nm.The powder X-ray diffraction indicated that PCH contained both MMT clay and MCM-41 and have located in the clay interlayers speaces.The diffuse reflectance spectra demonstrated the presence of CuO, Cu2O and Cu nanoparticles in PCH.
The nanocomposite (CuO-PCH) was used for antibacterial testing.The antibacterial activity of CuO-PCH nanocomposite was tested against Gram-positive and Hat Bands Gram-negative bacteria.The nanocomposite showed efficient bacterial effect against Gram-positive bacteria.The mechanism of antibacterial activity of CuO-PCH nanocomposite was discussed.